| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carsillo, Ava C (7)
|
ADDELM |
|
46.59 |
- |
| 2 |
Pedersen, Alexandra J (8)
|
ADBENN |
58.32 |
49.73 |
-8.59 |
| 3 |
Smith, Lena T (8)
|
NESNAP |
52.33 |
50.40 |
-1.93 |
| 4 |
Pecunia, Clara C (8)
|
ADBSPA |
57.10 |
51.21 |
-5.89 |
| 5 |
Kline, Keaghan E (8)
|
NESNAP |
53.71 |
54.95 |
1.24 |
| 6 |
Bourassa, Annalise (8)
|
ADSSCY |
|
58.26 |
- |
| 7 |
Deal, Grace A (7)
|
ADCLIN |
1:05.95 |
1:04.62 |
-1.33 |
| 8 |
Ahl, Grace E (8)
|
ADBSPA |
1:07.61 |
1:05.68 |
-1.93 |
| 9 |
Beckenstein, Sierra L (8)
|
ADBSPA |
|
1:10.04 |
- |
| 10 |
Kakaty, Mary C (7)
|
ADBSPA |
|
1:18.21 |
- |
|
Cronlund, Miranda M (8)
|
ADDELM |
48.83 |
NS |
- |
|
Huang, Rylie Z (7)
|
ADSSCY |
55.89 |
NS |
- |